How To Make Bromophenol Blue Solution - How To Make

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How To Make Bromophenol Blue Solution - How To Make. A 10μl sample can be loaded into the agarose gel well. Dissolve 0.4 g of bromocresol purple in 30 ml of water, add 6.3 ml of 0.1 m sodium hydroxide and dilute with water to make 500 ml (solution b).

The invention relates to a preparation method of bromophenol blue, which comprises the following steps: Dissolve the content by inverting the tube number of times or using a rotator/vortexer until all the ingredients are dissolved completely. Weigh accurately 40 mg of bromophenol blue and pour it into a 100.00 ml volumetric flask containing 01.20 ml of 0.05n sodium hydroxide solution and 10.00 ml of 95% ethanol, warm it. Bromophenol blue powder and methanol. All rights are available dna staining protocol for oligo. The dye is not very soluble. From the stock solution of the 6x gel loading dye, firstly, we have to prepare to work 1x dye and then we can use it for electrophoresis. A 10μl sample can be loaded into the agarose gel well. Classic recipes say 0.25% bromphenol blue (0.25g/100ml) in a solution containing a viscous substance like: = 0.010 grams of bromophenol blue in 20 ml of water makes 0.05% (mass/volume) bromophenol blue.

At neutral ph, the dye absorbs red light most strongly and transmits blue light. Add 20 ml of alcohol and dilute to 1 l with distilled water. So for 25μl of pcr product roughly add 5 to 7μl of dna gel loading dye to the pcr tubes. It is better to add dna gel loading dye directly to the pcr tubes containing our pcr amplicon. The solution should be deep blue. Weigh accurately 40 mg of bromophenol blue and pour it into a 100.00 ml volumetric flask containing 01.20 ml of 0.05n sodium hydroxide solution and 10.00 ml of 95% ethanol, warm it. Click to see full answer. = 0.010 grams of bromophenol blue in 20 ml of water makes 0.05% (mass/volume) bromophenol blue. The concentration of the prepared solution is about 0.4 g/l. Shake equal volumes of solution a, solution b and chloroform in a. 2) store at room temperature.